The main research theme in my laboratory is synthetic biology, which is an emerging field that covers wide range of areas, from the classical protein engineering to metabolic engineering. Since my Ph.D study, I have been interested in nucleic acid binding proteins as well as nucleic acids. Most works have focused on developing new tools to control gene expression and designing new proteins with novel functions. Currently research topics in my lab can be classified as follows:
Protein and Nucleic acid chemistry: Structural and functional study of protein-nucleic acid interaction.
Bioengineering: Developing tools for molecular biology and gene therapy.
Nanobio-Chemisty: Assembling nanostructures using biomaterials and inorganic materials.
Journal Articles
(2023)
Construction of a Z-DNA-Specific Recombinant Nuclease Zαα-FOK for Conformation Studies.
Methods in Molecular Biology.
2651,
1
(2023)
Plasmonic−Magnetic Active Nanorheology for Intracellular Viscosity.
NANO LETTERS.
23,
5
(2020)
Dual conformational recognition by Z-DNA binding protein is important for the B-Z transition process.
NUCLEIC ACIDS RESEARCH.
48,
22
(2020)
Protein-induced B-Z Transition is Kinetically Accelerated by Introducing Single-Stranded Regions.
BULLETIN OF THE KOREAN CHEMICAL SOCIETY.
41,
4
(2019)
PTP Inhibitor XIX Inhibits DUSP22 by Conformational Change.
BULLETIN OF THE KOREAN CHEMICAL SOCIETY.
40,
2
(2018)
Accelerated Development of Hippocampal Neurons and Limited Adhesion of Astrocytes on Negatively Charged Surfaces.
LANGMUIR.
34,
4
(2017)
Detection of Surrogate Cells Presenting Vaccinia Virus Protein by DNA Aptamers.
BULLETIN OF THE KOREAN CHEMICAL SOCIETY.
38,
1
(2016)
A bis(pyridine-2-ylmethyl)amine-based selective and sensitive colorimetric and fluorescent chemosensor for Cu2+.
SENSORS AND ACTUATORS B-CHEMICAL.
222,
1
(2015)
Structure and function of Zalpha, a Z conformation-specific nucleic acid binding domain
Structure and function of Zalpha, a Z conformation-specific nucleic acid binding domain.
Bio design.
3,
4
(2015)
Effect of polydimethylsiloxane (PDMS) coating on TiO2-based MALDI matrix for dimethyl methylphosphonate (DMMP) analysis.
APPLIED SURFACE SCIENCE.
353,
1
(2015)
Control of Microbial Growth in Alginate/Polydopamine Core/Shell Microbeads.
CHEMISTRY-AN ASIAN JOURNAL.
10,
10
(2015)
Direct Patterning and Biofunctionalization of a Large-Area Pristine Graphene Sheet.
CHEMISTRY-AN ASIAN JOURNAL.
10,
3
(2014)
Structural and biochemical characterizations of an intramolecular tandem coiled coil protein.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS.
455,
3-4
(2014)
Characterization of functional, noncovalently assembled zinc finger nucleases.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS.
453,
3
(2014)
Rapid Assembly and Cloning of Zinc Finger Proteins with Multiple Finger Modules.
BULLETIN OF THE KOREAN CHEMICAL SOCIETY.
35,
7
(2014)
Distinct Z-DNA binding mode of a PKR-like protein kinase containing a Z-DNA binding domain (PKZ).
NUCLEIC ACIDS RESEARCH.
42,
9
(2014)
Generation of Cellular Micropatterns on a Single- Layered Graphene Film.
MACROMOLECULAR BIOSCIENCE.
14,
3
(2013)
Energetics of Z-DNA Binding Protein-Mediated Helicity Reversals in DNA, RNA, and DNA-RNA Duplexes.
JOURNAL OF PHYSICAL CHEMISTRY B.
117,
44
(2013)
Z-DNA stabilization is dominated by the Hofmeister effect.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS.
15,
38
Publications
(2020)
생화학 Stryer.
범문에듀케이션.
Lead author
Patent/Intellectual Property
말디-질량분석용 유기매트릭스 용액의 제조방법 및 이에 의해 제조된 유기 매트릭스.
10-2015-0152263.
20170719.
KOREA, REPUBLIC OF
Homopiperazine compounds that inhibit ribosomal frameshifting by binding to RNA pseudoknot structure of sars coronavirus.
EP07123502.2.
20150304.
EU
Ｚ－ＤＮＡ 결합 단백질의 활성을 제어하는 피라졸리딘계 화합물 (PYRAZOLIDINE COMPOUND FOR CONTROLLING ACTIVITY OF Z-DNA BINDING PROTEIN).
1009939910000.
20101105.
KOREA, REPUBLIC OF
Homopiperazine compounds that inhibit ribosomal frameshifting by binding to RNA pseudoknot structure of sars coronavirus.
11/961,781.
20100928.
UNITED STATES
활성을 유지한 목표 단백질의 발현을 측정할 수 있는 재조합 유전자 발현 벡터 및 이의 이용 방법.
10-2008-0018561.
20100831.
KOREA, REPUBLIC OF
Conference Paper
(2019)
Protein engineering of the conformation-specific DNA binding protein.
한국구조생물학회 연례학술대회.
KOREA, REPUBLIC OF
(2019)
Altering the conformational specificity of DNA binding proteins.
The 33rd Annual Symposium of Protein Society.
UNITED STATES
(2019)
Protein-induced structural deviations of Z-DNA.
The 33rd Annual Symposium of Protein Society.
UNITED STATES
(2019)
Structural plasticity of Z-DNA conformation.
International Conference of the Korean Society for Structural Biology.
KOREA, REPUBLIC OF
(2019)
Effect of the MBP fusion on the target specificity of SpCas9.
KSBMB International Conference 2019.
KOREA, REPUBLIC OF
(2019)
Protein-induced Z-DNA formation of d(CCCGGG)₂ shows a significant structural plasticity.
KSBMB International Conference 2019.
KOREA, REPUBLIC OF
(2018)
The Zα-induced B-to-Z conversion rate is kinetically accelerated by introducing single-stranded regions..
대한화학회 제121회 학술발표회.
KOREA, REPUBLIC OF
(2017)
Engineering of a B-DNA Binding Protein to alter its DNA conformational specificity..
The 7th international meeting of on synthetic biology.
SINGAPORE
(2016)
Quantitatively controlled, self-regulating gene expression system.
Systems biology of gene regulation & genimic editing at the 2016 Cold Spring Harbor Asia Conference.
CHINA
(2016)
Engineering of conformation-specific proteins based on three-dimensional structue homology.
2016 한국구조생물학회 연례학술대회.
KOREA, REPUBLIC OF
(2015)
Protein-dependent destabilization of DNA duplex on DNA-linked AuNP assembly.
2015 International Chemical Congress of Pacific Basin Societies.
UNITED STATES
(2015)
DNA 압타머를 이용한 효모대리세포에서 발현된 백시니아 바이러스의 단백질 탐지.
2015 한국군사과학기술학회 추계학술대회.
KOREA, REPUBLIC OF
(2015)
Biochemical analysis of the recognition helix of Z-DNA binding proteins: Roles in conformational specificity.
The 29th annual symposium of the Protein Society.
SPAIN
(2015)
Aptamer-based detection of yeast surrogate expressing a vaccinia protein.
IFFM 2015.
KOREA, REPUBLIC OF
(2015)
DNA strand separation by proteins on DNA-linked AuNP particles.
IFFM 2015.
KOREA, REPUBLIC OF
(2014)
A new monitoring tool for the solubly expressed recombinant proteins based on the programmed alternative reading system.
2014 Cold spring harbor asia conference-Synthetic Biology.
CHINA
(2014)
Construction of fully functional noncovalently-assembled chimeric endonucleases.
2014 Cold spring harbor asia conference-Synthetic Biology.
CHINA
(2014)
Development of a Monitoring Tool for the Soluble Expression of Recombinant Proteins in Escherichia coli.
Asia-Pacific Conference on life science and engineering.
CHINA
(2014)
Development of quantitative In vivo assay system for non-specific DNA cleavages by zinc finger nuclease in yeast.
Asia-Pacific Conference on life science and engineering.
CHINA
(2014)
Expression of the recombinant vaccinia viral proteins in yeast: Dual-expression for localixation and identification.
Asia-Pacific Conference on Life Science and Engineering.
CHINA
